Identification of lung innervating sensory neurons and their target specificity in mouse (1)

Justinn Barr, Ph.D.
Jamie Verheyden, Ph.D.
Xin Sun, Ph.D.

Lightsheet imaging of mouse lung lobes

Updated on December 16, 2021 (Version 1, Revision 1)

Corresponding Contributor:

Jamie Verheyden
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Dataset Overview

Study Purpose: To map the innervation pattern of the mouse lung and projections from the nodose ganglia.

Data Collection: This dataset contains Zeiss LightSheet immunofluorescence imaging of neuronal markers in mouse lung tissue.

Conclusions: This sample dataset includes LightSheet imaging of whole lung lobes highlighting the nerve projections from the nodose ganglia and their relationship to the lung airways.

Curator's Notes

Experimental Design: Lung samples were obtained from healthy transgenic mice expressing tdTomato fluorescent vesicular glutamate 2 (VGluT2) or Nkx2-1GFP reporters. Lung tissue was fixed in 4% paraformaldehyde, PFA, and subjected to standard CUBIC clearing protocol. Cleared samples were imaged using a Zeiss Z.1 light sheet fluorescence microscope (LSFM).

Completeness: This dataset is part of a larger study: Foundational mapping of the neural circuits that control intrinsic lung function.

Subjects & Samples: Adult (n=6) transgenic mice between 8-12 weeks old were used in the study.

Primary vs. derivative data: Primary data is organized by the subject ID and contains LightSheet fluorescence imaging of neuronal markers in mouse lung tissue. The derivative folder contains image data (JPEG2000 and OME-TIFF) that was derived from primary images (.CZI). .CZI images were converted with 20:1 compression to JPEG2000 (.jpx) by MBF Bioscience for web streaming and visualization on the SPARC Data Portal. .CZI images were also converted with lossless compression to OME-TIFF (.tif) by MBF Bioscience.


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Publishing history

December 14, 2021
Originally Published
December 16, 2021 (Version 1)
Last Updated

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