Calcium imaging tension recording and pellet transit in mouse colon in response to stimulation of the pelvic nerve (PNS)

Study Purpose: This study was conducted to determine the effects of pelvic nerve stimulation of colon on colonic motility.

Data Collection: Calcium imaging of cholinergic and nitrergic neurons as well as ICC, tension recording of proximal middle and distal colon, and pellet transit through the colon.

Primary Conclusion: None stated; this is ongoing study.

Curator's Notes

Experimental Design: Adult mice of each sex were killed by isoflurane inhalation, and cervical dislocation, in accordance with the requirements of the Animal Ethics Committee at the University of Nevada, Reno. A ventral midline incision was made and the entire colon was excised and placed into a sylguard-lined dissection dish filled with oxygenated Krebs-Ringers solution. The colon was then gently flushed of its contents. For all experiments, the colon was perfused with 35-degree, oxygenated Krebs-Ringers for the duration of the experiment.

Completeness: This dataset is part of a larger study: "Comprehensive Structural and Functional Mapping of Mammalian Colonic Nervous System"

Subjects & Samples: Male (n=15) and female (n=14) adult transgenic and wild-type mice were used in this study.

Primary vs derivative data: The primary data is organized in folders by subject ID. Sample subfolders include either calcium imaging videos or, for a subset of samples, tension recordings as .scq files. The. avi microscope movies are of calcium transient and movement responses of the middle colon of ICC (ICC-GCaMP), cholinergic neurons (ChAT-GCaMP6f) and nitrergic neurons (nNOS-GCaMP6f). There is no derivative data folder.