Quantification of rat gastric enteroendocrine cells

Study Purpose: This study aims to characterize the anatomy and hormone content of endocrine cells within the mucosal lining of the stomach. The work provides a foundation for understanding how these cells regulate digestive functions and how they might be targeted to treat digestive and metabolic diseases.

Data Collection: This dataset contains images that convey quantitative information on the distributions of enteroendocrine cells (EEC), defined by the hormones they contain within the rat corpus and antrum. These data are used to construct patterns of colocalization between hormones and regional distribution in the rat gastric mucosa. Techniques employed include multiple label immunohistochemistry and high-resolution confocal microscopy.

Primary Conclusion: Unlike in other regions of the gut, most EECs in the stomach contains only a single hormone. The majority of gastric EECs are closed cells that do not come into direct contact with stomach contents.

Curator's Notes

Experimental Design: Animals were sacrificed and each subject's gastric antrum and corpus regions were harvested. Tissue samples were stained for 5-HT, Gastrin (Gas)/CCK, Gherlin(GHR), Histidine decarboxylase (HDC), Peptide YY (PYY), and somatostatin (SOM).

Completeness: This dataset is part of a larger study.

Subjects & Samples: This study used juvenile male Sprague-Dawley rats (n=14); stomach samples were derived from the antrum and corpus regions of the stomach.

Primary vs derivative data: Data in the primary and derivative folders are divided into folders for each subject, followed by stomach region, session and stain. Images for each antibody are presented both alone and merged into tif files. The derivative folder contains xlsx files with quantification information.

Important Notes: (1) Code. We found the manifest in the code folder is very helpful in understanding the code and its usage. (2) Docs. The Docs folder contains complete, derived figures. (3) Source folder. This folder contains czi formatted images

Code Availability: The code folder contains code and images which can be opened in ImageJ.