Scaffold map - Anterograde tracing of spinal afferent innervation in flat-mounts of the rat stomach

Study Purpose: The goal of this work is to map axon innervation data and its derived density field to an annotated generic rat stomach scaffold.

Data Collection: The generic rat stomach scaffold (DOI: 10.26275/iefx-c2qi) is created based on the general description and average dimensions of rat stomachs as measured by Powley et al. The data mapped in this dataset was derived from the original dataset (DOI: 10.26275/rmcz-jfoq), whereby axons and their terminal structures characterized using anterograde tracer Dextran Biotin injected into the left thoracic DRG T7-T11 are digitally traced from the flat-mounts of the whole rat stomach muscular wall.

Primary Conclusion: None stated

Curator's Notes

Experimental Design: Not applicable.

Completeness: Complete.

Subjects & Samples: Segmentation data derived from flat-mount imaging of 3 male Sprague Dawley rat stomachs (DOI: 10.26275/rmcz-jfoq) are used for this mapping.

Primary vs derivative data: The primary folder contains the mapping tool provenance data file needed to produce the scaffold map. The mapping tool will be accessible from the release download page on the SPARC Portal. The primary folder also contains the MAP-Client workflow and settings files to reproduce the files. The derivative folder contains JSON files that are used to generate a visualization of the scaffold map on the web portal.

Code Availability: Scaffold Mapping Tools