Mapping of intrinsic cardiac nervous system (ICN) neurons in a 3D reconstructed rat heart

Alison Moss

Clara Leung

James S. Schwaber

Jin Chen

Jonathan Gorky

Leonard Eisenman

Maci Heal

Navid Farahani

Rajanikanth Vadigepalli

Shaina Robbins

Sirisha Achanta

Steve England

Susan Tappan

Todd Huffman

Zixi (Jack) Cheng

The distribution of neurons in the intrinsic cardiac nervous system (ICN) were mapped and visualized in a 3D reconstruction of a male rat heart.

Updated on August 14, 2019 (Version 1)

Corresponding Contributor:

Clara Leung

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Dataset Overview

A normal male rat heart was fixed and whole-mount diffusion stained with Cresyl Echt Violet to visualize ICN neurons. The whole heart was sectioned and imaged with a Knife Edge Scanning Microscope (KESM) to produce an image stack. Cardiac anatomy was segmented and single neurons were mapped in the image stack with the Tissue Mapper software. The annotated heart anatomy and distribution of single neurons were then visualized in a 3D reconstructed model of the heart.

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About this dataset

Publishing history

August 14, 2019

Originally Published

August 14, 2019 (Version 1)

Last Updated

DOI

https://doi.org/10.26275/wox9-gqzp

Cite this dataset

Moss, A., Leung, C., S. Schwaber, J., Chen, J., Gorky, J., Eisenman, L., Heal, M., Farahani, N., Vadigepalli, R., Robbins, S., Achanta, S., England, S., Tappan, S., Huffman, T., & (Jack) Cheng, Z. (2019). Mapping of ICN Neurons in a 3D Reconstructed Rat Heart (Version 1) [Dataset]. SPARC Portal. https://doi.org/10.26275/WOX9-GQZP

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