Mouse genetic models to manipulate enterochromaffin cell activity - Murine Organoid ELISA

Koki Tohara, Ph.D.
David Julius, Ph.D.

Quantification of serotonin release from intestinal organoids isolated from genetically modified mice.

Updated on November 15, 2023 (Version 1, Revision 1)

Corresponding Contributor:

Koki Tohara
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Dataset Overview

Study Purpose: The purpose of this study was to determine if the transgenic mouse lines Pet1Flp;Tac1Cre;RC::PFTox and Pet1Flp;Tac1Cre;RC::FL-hM3Dq effectively inhibit or stimulate serotonin release.

Data Collection: 5-hydroxytryptamine (5-HT) measurements released from murine intestinal organoids using ELISA (enzyme-linked immunosorbent assay).

Primary Conclusion: The conclusion of this study was that PFTox reduced the stimulated serotonin release and DCZ stimulated serotonin release in hM3Dq organoids.

Curator's Notes

Experimental Design: Murine intestinal organoids isolated from wildtype, Pet1Flp;Tac1Cre;RC::PFTox, and Pet1Flp;Tac1Cre;RC::FL-hM3Dq mice were washed twice in ice-cold PBS + 0.1% BSA. Organoid pellets were reconstituted and incubated for 15 minutes in 100 µL Ringer's (140 mM NaCl, 5 mM KCl, 2 mM CaCl2, 2 mM MgCl2, 10 mM HEPES-Na, 10 mM D-glucose, pH 7.4) + 20 μM allyl isothiocyanate (AITC) for PFTox organoids and Ringer's + 1.7 μM deschloroclozapine (DCZ) for hM3Dq organoids. After 15 minutes, supernatant was collected. Collected supernatant was diluted into half with Ringer's, and serotonin concentration was measured using a serotonin ELISA kit (Rocky Mountain Diagnotics, cat#: BA E-5900R).

Completeness: This dataset is a part of a larger study: "Mouse genetic models to manipulate enterochromaffin cell activity."

Subjects & Samples: Male (n=3) adult transgenic mice were used in this study.

Primary vs derivative data: Primary data contains a summative spreadsheet with 5-hydroxytryptamine (5-HT) measurements released from murine intestinal organoids using ELISA. Sample IDs' are displayed internally within each spreadsheet. There is no derivative data folder.


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Publishing history

October 14, 2023
Originally Published
November 15, 2023 (Version 1)
Last Updated

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