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Molecular phenotype distribution of single rat intracardiac neurons

Alison Moss

Clara Leung

James Schwaber

Jin Chen

Jonathan Gorky

Maci Heal

Rajanikanth Vadigepalli

Sean Nieves

Shaina Robbins

Sirisha Achanta

Susan Tappan

Zixi Cheng

Intracardiac neuron (ICN) samples were collected from a female rat heart using laser capture microdissection. We acquired transcriptomics data for 154 genes from the 151 samples we collected. Images of sampled neurons allow for 3D reconstruction of data.

Updated on August 30, 2019 (Version 3)

Corresponding Contributor:

Alison Moss

2,156 Files

48.5 GB

200 Records

CC-BY-4.0

Dataset Overview

We developed an approach to appreciating the 3D organization of the ICN while at the same time permitting single cell transcriptomics and connectomics. Through serial cryostat sectioning of a cryopreserved heart with imaging of serially collected and stained sections, it is possible to reconstruct the 3D context and collect single neurons using laser microdissection. The transcriptional profiles of these isolated neurons can be determined down to single cell resolution and mapped back into the 3D context generated by stacking the serial images.

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About this dataset

Publishing history

July 19, 2019

Originally Published

August 30, 2019 (Version 3)

Last Updated

DOI

https://doi.org/10.26275/j3o2-49zk

Cite this dataset

Moss, A., Leung, C., Schwaber, J., Chen, J., Gorky, J., Heal, M., Vadigepalli, R., Nieves, S., Robbins, S., Achanta, S., Tappan, S., & Cheng, Z. (2019). Molecular Phenotype Distribution of Single Rat Intracardiac Neurons (Version 3) [Dataset]. SPARC Portal. https://doi.org/10.26275/J3O2-49ZK

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