Each set includes membrane potential records from a myenteric neuron of mouse proximal colon plus micrographs of cell body morphology, immunoreactivity for nNOS, calretinin (where possible), and micrographs showing axonal projections
updated on August 9, 2022
microscopy, electrophysiology, immunohistochemistry
Microstructural analysis of food intake as a behavioral endpoint to determine the effect of chronic gastric electrical stimulation on the consumption of a high fat diet by adult male Sprague-Dawley rats.
This study aims to evaluate the effects of cervical VNS on gastric motility in rats. The aim is to selectively activate or block the afferent pathway to disentangle their differential effects on gastric responses.
We used MRI to map gastric electrical activation following meal consumption. Manganese ions were used as a surrogate of calcium ions to map the increase of calcium influx, presumably into smooth muscle cells.
updated on July 28, 2022
manganese-enhanced mri, gastric electrical activation, rat
Microstructural analysis of food intake was used as a behavioral endpoint to determine the effect of chronic gastric electrical stimulation on the consumption of a 45% high fat diet by adult female Sprague-Dawley rats.
Comprehensive mapping of lower urinary tract afferent projections within the lumbosacral spinal cord of male and female rats, using 3D reconstruction of sectioned cord (TissueMaker) and cleared intact cord (iDisco, light sheet microscopy).
updated on July 21, 2022
visceral afferent, motoneuron, tissue clearing
Comparison of cuff, microelectrode, and intrafascicular electrode to make evoked compound action potential (ECAP) and naturally occurring activity recordings from the peripheral nervous system. Dataset from pigs (n=3) - a human-scale large animal model.
We used immunohistochemistry to visualize the expression of molecular markers in stellate ganglia from mice and human. Data consist of images (CZI format) of SG cryosections stained with antibodies combinations, acquired with a Zeiss 710 microscope.
The generated body scaffold was fitted to data and the organ fiducial markers were embedded in the rat whole-body. The landmarks were obtained from image data and also were defined in the body scaffold.
The generated body scaffold was fitted to data and the organ fiducial markers were embedded in the mouse whole-body. The landmarks were obtained from segmenting MRI data and also were defined in the body scaffold.
Immunohistochemistry revealed the morphology and distribution of nociceptor Substance P-immunoreactive (SP-IR) axons and terminals in the muscular layers (longitudinal muscle, myenteric plexus, and circular muscle) of the mouse stomach.
updated on July 12, 2022
longitudinal and circular muscles, nociceptive afferents, confocal microscopy