Human vagus nerve stained with Masson's trichrome

Study Purpose: To collect samples of human vagus nerves at the cervical and subdiaphragmatic levels and provide Masson’s trichrome-stained cross sections

Data Collection: We collected 18 left cervical, 19 right cervical, 16 anterior subdiaphragmatic, and 16 posterior subdiaphragmatic vagus nerve samples from embalmed human cadavers and placed them in 4% PFA. Following paraffin embedding, we stained transverse cross sections with Masson’s trichrome. The dataset contains nd2 (filetype for Nikon’s NIS-Elements software) and TIFF files.

Primary Conclusion: None stated

Curator's Notes

Experimental Design: Cervical and/or subdiphragmatic vagus nerves were sampled from adult embalmed human cadavers (male and female), and each sample underwent paraffin embedding, transverse sectioning, and Masson’s trichrome staining.

Completeness: This dataset is part of a larger study "Quantified vagus nerve morphology across species".

Subjects & Samples: Adult male (n=15) and female (n=15) embalmed human cadavers were used for this study, 54 - 90+ years old. The dataset contains 18 left cervical, 19 right cervical, 16 anterior subdiaphragmatic, and 16 posterior subdiaphragmatic human vagus nerve samples.

Primary vs derivative data: Data in the primary and derived files are divided by subject identification, then sample number (listed in sample file). The primary folder contains nd2 and TIFF images. Image data (.jp2) was derived from primary images (.nd2) in Primary folder. Images were converted with 40:1 compression to .jp2 by MBF Bioscience for web streaming and visualization on the SPARC Data Portal.

Important Notes: (1) Fiji software can be used to open nd2 files (https://imagej.net/Fiji/Downloads) > Open Fiji > Plugins (top of the screen) > Bio-Formats > Bio-Formats Importer > Select the file.