Human vagus nerve anatomical reconstruction using microCT immunohistochemistry and ultrasound - f006

Study Purpose: With its large numbers of afferent (sensory) and efferent (motor) fibers, the vagus is the main conduit for bidirectional communication between the brain and visceral organs and participates in autonomic reflexes regulating cardiorespiratory, gastrointestinal, and neuroimmune functions. In the human vagus, nerve fibers are arranged in fascicles. Along the vagus, afferent and efferent fibers leave the fascicles and emerge from the nerve trunk to form branches, which in turn provide sensory and motor innervation to essentially all visceral organs in the neck, chest and abdomen. Even though much is known about the macroscopic and microscopic anatomy of the vagus, the spatial organization of fascicles and fibers within the nerve, as it relates to the innervated organs and the sensory and motor functions of the vagus, is largely unknown. The spatial organization of fibers in the human vagus has implications for vagus neuromodulation therapies. The overall objective of this proposal is to create and share with the scientific community a quantified map of the fascicular and microscopic structure and the organ connectivity of the human vagus nerve, from the brainstem to the abdominal region, with several cross-registered layers of anatomical information at the organ branch, fascicle, and single fiber level.

Data Collection: The dataset includes anatomical images and videos, Ultrasound images and videos of the vagus nerve, microCT scans across the length of the nerve and IHC image across multiple levels. The techniques include : anatomy (dissection), histology (immunohistochemistry), bioimaging (ultrasound ) and microscopy (microCT)

Primary Conclusion: The human vagus nerve exhibits an intricate anatomical organization. This is an ongoing study, and we will publish our initial conclusions once we have sufficient data.

Curator's Notes

Experimental Design: Cadavers were embalmed with formalin via the femoral artery. Dissection of the vagus nerve began with exposing the cervical vagus from the anterior direction from the level of the angle of the mandible to the level of the clavicle. Next, the vagus nerve was dissected in the thorax, followed by the abdomen. Finally, the vagus nerve was dissected in the superior cervical region beginning at the inferior border of the jugular foramen. Vagal branches were identified based on their target tissues and then marked with tissue dye and/or sutures. Video and photographic documentation of the dissection process was taken.

Ultrasound: Ultrasound images of the nerves were collected during dissection and measurements in 2D planes were recorded. 2D images were saved and 3D volumetric reconstructions were performed from these images.

Micro-CT:The nerves were then cut into 1.5 cm segment and transferred to lugol solution, where they stayed for 1 week. Micro-CT imaging was performed after wrapping the samples with sarene wrap to prevent dehydration. A bruker micro-CT Skyscan machine was used for micro-CT imaging, with a voxel size of 9 microns was used, for optimal resolution and imaging time balance.

Histology/IHC/staining:The segments were then transferred for IHC staining to the Feinstein institute, where the samples were cut into sub-segments of 0.5 cms, dehydration performed with ethanol and xylene. From each of the sub-segments, we determined 5 levels, starting from the most proximal end, with 1000 micron intervals, to the distal end. At every level, 5 consecutive 5 micron thick sections were taken, one of the sections was used for Immunohistochemistry staining and one for H&E staining, the remaining 3 sections were taken as backup/duplicate sections.

After sectioning, slides were subjected to deparaffinization using xylene and rehydrated with ethanol rinse, and washed with distilled water, stained with NF (neurofilament), MBP (myelin basic protein), TH (tyrosin hydroxilase) and ChAT (choline acetyl transferase) stains. And imaged with a BZ-X800 all in one flourescence microscopy, the H&E images were imaged with bright field microscopy.

Completeness: This is a part of a series of a dataset created as a part of SPARC REVA reward: "Human vagus nerve anatomical reconstruction using microCT immunohistochemistry and ultrasound". This dataset is incomplete, more data is expected to be uploaded in the future. Protocols will be made available in a later time.

Subjects & Samples: 1 human female cadaver. Samples: 34 segments, 27 subsegments, 27 sections, and 60 sites.

Primary vs derivative data: Samples in the primary data are organized in a hierarchical fashion, with left and right nerves being the top level of samples. From these left and right nerves, the segments are the next highest level of organization derived from the dissection. MicroCT imaging is then performed on the segments. The segments were cut into sub-segments of 0.5 cms, and from each of the sub-segments, we determined 5 levels, starting from the most proximal end, with 1000 micron intervals, to the distal end. At every level, 5 consecutive 5 micron thick sections were taken. One of the sections was used for immunohistochemistry staining and one for H&E staining, with the remaining 3 sections taken as backup/duplicate sections. Derivative data contains processed MicroCT and IHC data, and also contains the output fascicle and fiber quantification pipelines.

Important Notes: Preliminary data release to test publishing and processing pipelines. This is an ongoing project; more data is expected in the future.