Bulk proteomics of naive human dorsal root ganglion

Study Purpose: This study set out to a) provide a quantitative proteomic reference for the human DRG and nerve root and b) explore sexual dimorphism in these tissues.

Data Collection: DIA-PASEF proteomics was performed on hDRG tissue from donors.

Primary Conclusion: Multi-omic integration with human DRG proteomics highlights TNFa signaling as a relevant sexually dimorphic pathway in the periphery.

Curator's Notes

Experimental Design: Lumbar human dorsal root ganglia (hDRGs) were surgically excised from donors and immediately frozen in powdered dry ice before storage at -80°C. Samples were transported and stored under cryogenic conditions prior to proteomic analysis. Tissue preparation utilized a single-pot, solid-phase-enhanced sample preparation (SP3) method, followed by peptide analysis using Nano-RPLC coupled with a mass spectrometer (timsTOF HT in DIA) for detailed protein profiling.

Completeness: This dataset is part of a larger study, "hDRG proteomics"

Subjects & Samples: Samples from male (n=4) and female (n=4) human donors were used in this study.

Primary vs derivative data: The primary data folder is organized by subject and sample name and contains *-.d outputs from a timsTOF HT mass spectrometer. A folder LC-QC files, which contain pooled QC samples for LC-MS/MS quality control, and SP3-QC files, which are repeat samples used to verify quality across SP3 rounds (refer to the full protocol for details). The derivative data folder contains a report.tsv file generated by DIA-NN-1.8.1 with match-between-runs enabled, a report.log.txt file that provides the output log for report.tsv generation, and a metadata.csv file with relevant column data.

Important Notes: This dataset will also be made searchable through the UTDallas sensoryomics site.

Code Availability: code is available https://github.com/aliibarry/hDRG-proteomics