Single cell RNA sequencing from human dorsal root ganglion

Study Purpose: The purpose of the study is to utilize the Single Cell Gene Expression FLEX kit from 10X Genomics to identify and characterize cellular populations and gene expression profiles at a single-cell resolution from human dorsal root ganglion. This approach allows for a comprehensive understanding of the cellular heterogeneity within a complex tissue at a whole cell level.

Data Collection: Lumbar dorsal root ganglia (DRGs) were obtained from human organ donors with no known history of chronic pain, in collaboration with the Southwest Transplant Alliance. Following surgical extraction, the DRGs were immediately placed in chilled, oxygenated artificial cerebrospinal fluid (aCSF). To isolate whole cells, the tissue underwent enzymatic digestion, followed by density gradient centrifugation to remove non-cellular debris. The isolated cells were then fixed and hybridized with probes provided by 10X Genomics, after which library preparation and sequencing were conducted.

Primary Conclusion: We were able to characterize cellular populations and gene expression profiles at a single-cell resolution from human dorsal root ganglion.

Curator's Notes

Experimental Design: Human dorsal root ganglia (DRGs) from lumbar levels L3-L5 were surgically extracted from organ donors with no history of chronic pain at the Southwest Transplant Alliance (STA) 2-4 hours post-cross-clamp. The DRGs were immediately placed in chilled, oxygenated aCSF and transported to the University of Texas at Dallas for further processing. Single cells from the DRGs were isolated, and the cell pellet was fixed using the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (1000414). Single-cell RNA sequencing was then performed using the Single Cell Gene Expression FLEX kit from 10X Genomics.

Completeness: This dataset is part of a larger study, " Single-cell RNA sequencing of dorsal root ganglia from human organ donors."

Subjects & Samples: Samples from male (n=2) and female (n=1) human donors were used in this study.

Primary vs. derivative data: The Primary data folder is organized by subject and sample name and contains Cell Ranger pipeline output. Sequencing data were processed and mapped to the human (GRCh38) genome using 10X Genomics Fix RNA profiling Cellranger multi pipeline (v7). This dataset does not contain raw sequencing data. There is no derivative data folder.