Myenteric neuron activity during spontaneous motor complexes in mouse colon

Study Purpose: The objective of this study was to record myenteric neuron activity during spontaneous motility behavior.

Data Collection: Calcium imaging in full-length, full-thickness mouse colon.

Data Conclusion: Our data reveal unique patterns of activity that occur from individual myenteric neurons prior to initiation of motor complexes, which regularly occur every 2-5 minutes.

Curator's Notes

Experimental Design: Entire colons were removed from E2a-GCaMP6s mice, cut open longitudinally, and pinned flat with the mucosal side facing down, in a Sylgard-lined dish, superfused with oxygenated artificial cerebrospinal fluid (ACSF). The intact full-length, full-thickness colons were transferred to the stage of an upright DM6 FS Leica fluorescent microscope equipped with Prime 95B Scientific Complementary Metal-Oxide-Semiconductor (CMOS) camera, 10X objective lens, and Metamorph software (RRID:SCR_002368). The ACSF was slowly heated up to 35-37 C. Several consecutive 3-minute movies were collected for 9-15 minutes to capture multiple CMCs. The imaging parameters were set up to 150 ms exposure time, 1200 frames, and 2x2 binning.

Completeness: This dataset is complete.

Subjects & Samples: Male (n=1) and female (n=3) transgenic mice, 4–6 months old were used in this study. E2a-Cre mice (RRID:IMSR_JAX:003724) were crossed with mice containing a floxed-STOP-GCaMP6s sequence in the Rosa26 locus (Ai96 mice, RRID:IMSR_JAX:028866).

Primary vs derivative data: Primary data is organized by subject ID and contains imaging of calcium dynamics (GCaMP) in the mouse colon as 1200 frames image stack .tif file. Ther is no derivative data folder.