Current clamp recordings of action potential responses to norepinephrine, neuropeptide Y, acetylcholine, and vasoactive intestinal peptide.
Study Purpose: Sympathetic and parasympathetic stimulation play an essential role in regulating the electrical properties of ventricular myocytes in the heart. Norepinephrine (NE) is the the primary neurotransmitter associated with sympathetic responses, while acetylcholine (ACh) is the primary neurotransmitter associated with parasympathetic responses. However, both sympathetic and parasympathetic neurons are known to release other neurotransmitters that may contribute to functional responses. The focus of this study was to examine the effect that potential cotransmitters have on action potentials recorded from ventricular myocytes isolated from the pig heart. We examined the effects of the sympathetic cotransmitter neuropeptide Y (NPY) alone and in combination with NE, as weil as the effects of the parasympathetic cotransmitter vasoactive intestinal peptide (VIP) alone and in combination with ACh.
Data Collection: Membrane potentials were recorded from isolated pig ventricular myocytes using the whole-cell configuration of the patch clamp technique.
Primary Conclusion: This information is meant to be used in generating computational models that are able to accurately predict mechanisms contributing to autonomic effects on cardiac function in the pig heart.
Curator's Notes
Experimental Design: Membrane potentials were recorded from isolated pig ventricular myocytes using the whole-cell configuration of the patch clamp technique. Isolated cells were placed in a perfusion chamber on the stage of an inverted microscope and bathed in an extracellular solution, then patched using microelectrodes with resistances between 1 and 2 MΩ. Membrane potential was recorded under current clamp conditions using a Multiclamp 700B voltage clamp amplifier, Digidata 1440A computer interface, and pClamp 11 data acquisition and analysis software (RRID:SCR_011323). Data were lowpass filtered at 4 kHz, and sampled at 10 kHz.
Completeness: This dataset is complete.
Subjects & Samples: Female (n=9) and male (n=23) adult domestic pigs were used in this study.
Primary vs derivative data: Primary data is organized by the subject ID and contains raw recordings from individually collected cells (sample ID) as .adf files. There is no derivative data folder.
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Harvey, R., & Agarwal, S. (2020). Isolation of Adult Pig Ventricular Myocytes v1. https://doi.org/10.17504/protocols.io.ba8iihue
Harvey, R., & Agarwal, S. (2020). Cardiac Action Potential Protocol v1. https://doi.org/10.17504/protocols.io.ba8qihvw