Microstructural analysis of food intake was used as a behavioral endpoint to determine the effect of chronic gastric electrical stimulation on the consumption of a 45% high fat diet by adult female Sprague-Dawley rats.
Study Purpose: Little is known about the ability of chronic gastric electrical stimulation (GES) to alter the feeding behavior and body weight of adult female Sprague-Dawley rats. Thus, we paired continuous GES with the presentation of a high-fat diet, which is known to result in obesity, and recorded food intake along with body weight for six weeks.
Data Collection: Microstructural analysis of food intake was used as a behavioral endpoint to determine the effect of chronic gastric electrical stimulation on the consumption of a 45% high fat diet by adult female Sprague-Dawley rats.
Primary Conclusion: Stimulated and unstimulated female rats did not differ in body weight and food intake throughout the six-week study. At the end of the study, both groups did not differ in body fat content and blood glucose levels.
Experimental Design: Twenty-four adult female Sprague-Dawley rats were each implanted with a patch electrode at a location on the forestomach wall that is known to contain a high density of vagal afferent terminals that are thought to play a role in the monitoring of gastric compliance and gastric motility. Fourteen female rats were continuously stimulated for 6 wks (0.6mA, 0.2ms, 5Hz, 20s On:40s Off) while the remaining ten female rats served as unstimulated shams. Gastric electrical stimulation was paired with the presentation of a 45% high-fat diet (Research Diets, D12451). The feeding behavior of individually housed rats was continuously monitored throughout the duration of the study using the BioDAQ automated intake monitoring system from Research Diets. Body Fat Measurement: Following completion of the study, body fat content for each rat was determined using an EchoMRI-900 Quantitative Magnetic Resonance Body Composition Analyzer (Echo Medical Systems, LLC). Blood glucose was measured following a 6-hour fast. The fast was started in the morning at the onset of the 12h lights-on period of the light:dark cycle.
Completeness: This dataset is complete.
Subjects & Samples: Adult female (n=24) Sprague-Dawley (RRID:RGD_737903) were used in this study.
Primary vs derivative data: The primary data consist of a recording of feeding behavior for individual subjects collected over the course of the experiment in a spreadsheet XLSX format. The derivative folder contains the transformed primary data located in the primary folder. The data consist of summative feeding data in a multiworkbook spreadsheet and the endpoint measurement of body fat and blood glucose levels. Thumbnail graphs of the transformed data are included. As back mount failure occurred starting six to eight weeks post-surgery, only the data from experimental weeks completed (i.e., the full seven days) prior to failure was used in the derivative excel files and companion thumbnails.
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