Increased arrhythmia susceptibility in type 2 diabetic mice related to dysregulation of ventricular sympathetic innervation

Study Purpose: Investigates neural control of heart in control and type 2 diabetic mice.

Data Collection: Dataset includes intracellular microelectrode recordings from control and diabetic mouse intrinsic cardiac neurons and stellate ganglion neurons.

Primary Conclusion: Increased arrhythmia susceptibility in type 2 diabetic mice related to dysregulation of ventricular sympathetic innervation

Curator's Notes

Experimental Design: Intrinsic cardiac or stellate ganglia were isolated from adult male mice under deep isoflurane (5%) anesthesia. Ganglion preparations were superfused continuously (6–7 mL/min) with physiological salt solution (32–35°C). Individual neurons were impaled using microelectrodes filled with either 2M KCl (60–120 MΩ) or 2M KCl + 2% neurobiotin (80–160 MΩ; Vector Laboratories). Membrane voltage was recorded using a Multiclamp 700B amplifier coupled with a Digidata 1550B data acquisition system and pCLAMP 10 software (Molecular Devices, Sunnyvale, CA).

Completeness: This dataset is a part of a larger study: "IC neurons: passive, active and synaptic evoked membrane properties"

Subjects & Samples: Male (n=16) mice (RRID:IMSR_JAX:000642) were used in this study.

Primary vs derivative data: Primary data is organized in folders by subject ID. Each subject folder contains intracellular microelectrode recording in axon binary files .abf