Human vagus nerve TH- (tyrosine hydroxylase) and ChAT- (choline acetyltransferase) positive fibers

Study Purpose: This dataset provides immunohistological images of cross sections of human vagus nerves, identifying choline acetyl transferase positive, ChAT+, and tyrosine hydroxylase positive, TH+, fibers, thereby providing information on the quantity and localization of parasympathetic and sympathetic efferent fibers within the vagus nerve.

Data Collection:The dataset contains image files (TIF format) for 9 left cervical and 9 anterior subdiaphragmatic human vagus nerve samples, each labeled with one of two antibodies: anti-ChAT or anti-TH, totaling ~7 GB. Section stains were interleaved, with anti-ChAT then anti-TH on successive sections.

Primary Conclusion: None stated.

Curator's Notes

Experimental Design: Slides were prepared from paraffin-embedded vagus nerve, stained with the appropriate antibodies/fluorophores and imaged. In this dataset, the nerves were stained to identify ChAT+ and TH+ nerve fibers.

Completeness: Complete.

Subjects & Samples: This dataset contains 23 samples of either the left cervical nerve or anterior subdiaphragmatic vagus nerve from 16 human subjects.

Primary vs derivative data: Primary data is a set of microscopy images in TIF format. Derivative data is formatted as JP2 (also known as JPEG 2000) in addition to TIF.