Human vagus nerve TH- (tyrosine hydroxylase) and ChAT- (choline acetyltransferase) positive fibers

Nicole A Pelot, Ph.D.
,
J Ashley Ezzell
,
Jake E Cariello
,
Gabriel B Goldhagen
,
Kara A Clissold
,
Warren M Grill

This dataset provides immunohistological images of cross sections of human vagus nerves, identifying ChAT+ and TH+ fibers.

Updated on June 23, 2022 (Version 1, Revision 1)

Corresponding Contributor:

Nicole Pelot
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15.86 GB
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Dataset Overview

Study Purpose: This dataset provides immunohistological images of cross sections of human vagus nerves, identifying choline acetyl transferase positive, ChAT+, and tyrosine hydroxylase positive, TH+, fibers, thereby providing information on the quantity and localization of parasympathetic and sympathetic efferent fibers within the vagus nerve.

**Data Collection:**The dataset contains image files (TIF format) for 9 left cervical and 9 anterior subdiaphragmatic human vagus nerve samples, each labeled with one of two antibodies: anti-ChAT or anti-TH, totaling ~7 GB. Section stains were interleaved, with anti-ChAT then anti-TH on successive sections.

Primary Conclusion: None stated.


Curator's Notes

Experimental Design: Slides were prepared from paraffin-embedded vagus nerve, stained with the appropriate antibodies/fluorophores and imaged. In this dataset, the nerves were stained to identify ChAT+ and TH+ nerve fibers.

Completeness: Complete.

Subjects & Samples: This dataset contains 23 samples of either the left cervical nerve or anterior subdiaphragmatic vagus nerve from 16 human subjects.

Primary vs derivative data: Primary data is a set of microscopy images in TIF format. Derivative data is formatted as JP2 (also known as JPEG 2000) in addition to TIF.

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About this dataset

Publishing history

April 21, 2022
Originally Published
June 23, 2022 (Version 1)
Last Updated

Cite this dataset

Pelot, N. A., Ezzell, J. A., Cariello, J. E., Goldhagen, G. B., Clissold, K. A., & Grill, W. M. (2022). Human vagus nerve TH- (tyrosine hydroxylase) and ChAT- (choline acetyltransferase) positive fibers (Version 1) [Dataset]. SPARC Portal. https://doi.org/10.26275/X10I-9C9U

References

Is Supplemented by

Ashley Ezzell, J., A. Pelot, N., A. Clissold, K., & M. Grill, W. (2019). SPARC_Duke_Grill_OT2-OD025340_VagusNerve_IHC_TH v1. https://doi.org/10.17504/protocols.io.6hehb3e

Ashley Ezzell, J., A. Pelot, N., A. Clissold, K., & M. Grill, W. (2019). SPARC_Duke_Grill_OT2-OD025340_VagusNerve_IHC_ChAT v1. https://doi.org/10.17504/protocols.io.6hfhb3n

Ashley Ezzell, J., A. Pelot, N., A. Clissold, K., & M. Grill, W. (2020). SPARC_Duke_Grill_OT2-OD025340_VagusNerve_IHC_ChAT v2. https://doi.org/10.17504/protocols.io.bi9tkh6n