Single nucleus RNAseq of nodose ganglia in mice

Study Purpose: The goal of this proposed work is to illustrate the neural circuit that originates from the lung and returns to the lung. Current knowledge indicates that the pulmonary neural circuit controls breathing, immune responses, pulmonary blood pressure, and possibly other key aspects of fundamental physiological activities, many of which are critically disrupted in diseases. We anticipate that our findings will contribute to the foundation for using neuromodulatory therapies as a realistic option to improve the effectiveness and specificity of treatment.

Data Collection: The afferent, as well as efferent neurons that innervate all three cell types in lung, the PNECs, and the airway and vascular smooth muscles, are sequenced. For a single sample, we dissected nodose ganglia from mice and pooled them together for douncing and nucleus isolation, sorting, and sequencing.

Conclusions: None stated

Curator's Notes

Experimental Design: Nodose ganglia were dissected and flash-frozen in liquid nitrogen. Stored at -80C until ready to isolate single nuclei from nodose vagal ganglia neurons in preparation for 10xGenomics library prep protocol.

Completeness: This dataset is part of a larger study: "Foundational mapping of the neural circuits that control intrinsic lung function".

Subjects & Samples: Adult male mice (n=2) between 8-10 weeks old were used in the study.

Primary vs derivative data: Primary data is organized by the subject ID, each subject folder contains raw RNA-Seq FastQ files. Derivative data folder contains sequencing sumary files listing barcodes, gene names, and gene matrixes.