This dataset contains baseline measurements of mouse stellate ganglia using RNAseq
Study Purpose: The stellate ganglia are the predominant source of sympathetic innervation to the heart. Remodeling of the nerves projecting to the heart has been observed in several cardiovascular diseases, however, studies of adult stellate ganglia are limited. A profile of the baseline transcriptomic and neurochemical characteristics of the stellate ganglia in adult C57Bl6J mice, a common model for the study of cardiovascular diseases, may aid future investigations
Data Collected: In this study, RNA-sequencing is used to identify expression differences between stellate ganglia in male and female mice. RNA-sequencing analysis of dataset derived from https://doi.org/10.1038/s41598-018-27306-3 and https://www.ncbi.nlm.nih.gov/sra/SRX3577001[accn]. The analysis contains differential expression analysis and gene ontology analysis of these differences included physiologically important genes for growth factors, receptors, and ion channels.
Primary Conclusion: While the neurochemical profiles of male and female stellate ganglia were not different, minor differences in neurotransmitter content were identified in heart tissue.
Experimental Design: Left stellate ganglia were removed from 16-19 weeks old mice. The clean stellate ganglia were chopped, triturated, digested in RNeasy RLT buffer, and RNA was extracted using the RNAqueous Micro kit (Ambion) according to the manufacturer’s instructions. Six biological replicates for female (n=6) and male (n=6) stellate ganglia samples were contrasted by high throughput sequencing. Sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with RiboZero ribosomal RNA removal (Illumina), using 130 ng of RNA per sample. Sequencing was performed using an Illumina HiSeq 2500 through the Massively Parallel Sequencing Shared Resource at Oregon Health & Science University: Portland, Oregon, US (OHSU).
Completeness: This dataset is complete.
Subjects & Samples: Male (n=6) and female (n=6) C57BL/6J mice age between 16-19 weeks were used in the study.
Primary vs derivative data:. There is no primary data included in this dataset. Analyzed data are listed in the derivative folder as a tabularized gene expression summary. Original fastq raw data is available online at https://www.ncbi.nlm.nih.gov//bioproject/PRJNA423086
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