3D imaging of enteric neurons in mouse

Study Purpose: To examine intra-interganglionic connectivity, neurochemical code, and morphology.

Data Collection: This dataset contains confocal projections of mouse proximal colon.

Primary Conclusion: None stated

Curator's Notes

Experimental Design: Mice were anesthetized, surgery performed, the distal colon dissected and fixed in PFA for 24 hours. Whole-mount stained preparations were imaged via confocal microscopy. A complete list of antibodies used is provided in the docs folder. Individual Z plane images by the channel labeling were collected.

Completeness: This dataset is part of a larger study: "Quantitative Morphology, Transcriptomics, and Function of Enteric Neurons Expressing VIP"

Subjects & Samples: This study used 57 adult and 2 embryonic mice of mixed genetic backgrounds.

Primary vs derivative data: The primary folder contains raw confocal images (.lif) collected organized by the animal number folders and respective sample numbers. The derivative folder contains converted primary microscopy images.

Important Notes: "Docs" folder contains essential information and a series of snapshots and tutorials allowing to understand the dataset better.