3D imaging of enteric neurons in mouse

Marthe Howard, Ph.D.

This dataset contains confocal projections of mouse proximal colon.

Updated on December 1, 2021 (Version 1, Revision 1)

Corresponding Contributor:

Marthe Howard
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Dataset Overview

Study Purpose: To examine intra-interganglionic connectivity, neurochemical code, and morphology.

Data Collection: This dataset contains confocal projections of mouse proximal colon.

Primary Conclusion: None stated


Curator's Notes

Experimental Design: Mice were anesthetized, surgery performed, the distal colon dissected and fixed in PFA for 24 hours. Whole-mount stained preparations were imaged via confocal microscopy. A complete list of antibodies used is provided in the docs folder. Individual Z plane images by the channel labeling were collected.

Completeness: This dataset is part of a larger study: "Quantitative Morphology, Transcriptomics, and Function of Enteric Neurons Expressing VIP"

Subjects & Samples: This study used 57 adult and 2 embryonic mice of mixed genetic backgrounds.

Primary vs derivative data: The primary folder contains raw confocal images (.lif) collected organized by the animal number folders and respective sample numbers. The derivative folder contains converted primary microscopy images.

Important Notes: "Docs" folder contains essential information and a series of snapshots and tutorials allowing to understand the dataset better.

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About this dataset

Publishing history

November 9, 2021
Originally Published
December 1, 2021 (Version 1)
Last Updated

Cite this dataset

Howard, M. (2021). 3D imaging of enteric neurons in mouse (Version 1) [Dataset]. SPARC Portal. https://doi.org/10.26275/9FFG-482D

References

Is Supplemented by

Howard, M. (2019). Wholemount Immunolabeling for GUT Samples v1. https://doi.org/10.17504/protocols.io.wr6fd9e

Described by

Nestor-Kalinoski, A., Smith-Edwards, K. M., Meerschaert, K., Margiotta, J. F., Rajwa, B., Davis, B. M., & Howard, M. J. (2022). Unique Neural Circuit Connectivity of Mouse Proximal, Middle, and Distal Colon Defines Regional Colonic Motor Patterns. Cellular and Molecular Gastroenterology and Hepatology, 13(1), 309-337.e3. https://doi.org/10.1016/j.jcmgh.2021.08.016