Optogenetic iBAT (interscapular brown adipose tissue) stimulation in anesthetized mice

Study Purpose: To examine functional effects of whole-body and iBAT (interscapular brown adipose tissue)-specific stimulation while under acute anesthesia.

Data Collection: Rectal temperature, iBAT temperature were collected.

Primary Conclusion: Optogenetic experiment did not show any indication that specific changes in iBAT temperature were produced beyond laser heating of iBAT tissue.

Curator's Notes

Experimental Design: Peripheral viral AAV (Adeno-associated virus) injection of 2.5 µL into the right iBAT (interscapular brown adipose tissue) pad and 2.5 µL injections into the left iBAT pad were administered to anesthetized animals. After recovery from the first surgery, the emitter transponder was implanted to allow for monitoring and recording of physiological data. Rectal temperatures, and iBAT temperatures were collected in two experimental groups: virally induced ChR2 expression and breeding induced ChR2 expression.

Completeness: This dataset is a part of a larger study: "Genetically-based neuro-modulation of adipose tissue functions".

Subjects & Samples: Male (n=11) and female (n=9) adult mice of mixed genetic background were used in the study.

Primary vs derivative data: The primary folder contains excel files where temperature recordings are presented in tabular form for all animals used in the study. Subject names are shown internally in each table. There is no derivative data folder.

Important Notes: This dataset is currently undergoing image registration and will be updated once this process is complete.