Identification of the gene expression profile of interscapular brown fat (iBAT)-related ganglia (SG/T1 & T3) and inguinal white fat (iWAT)-related ganglia (T13/L1 & L2).
Study purpose: The study goal is to identify the gene expression profile of interscapular brown fat (iBAT)-related ganglia (SG/T1 & T3) and inguinal white fat (iWAT)-related ganglia (T13/L1 & L2).
Data collection: RNA sequencing raw data in fastq, bz2 files.
Primary conclusion: Results from RNA sequencing data revealed that there were no gender differences in iBAT-related ganglia and iWAT-related ganglia. Additionally, we verified several known pre- and postganglionic signaling genes. We found robust differences between iBAT- and iWAT-related ganglia gene expression.
Experimental Design: Total RNA was extracted from the dissected interscapular brown adipose tissue (iBAT) related ganglia and inguinal white adipose tissue (iWAT)-related ganglia by Trizol RNA extraction protocol. Stellate ganglion (SG), thoracic ganglion T3, thoracolumbar ganglion T13/L1, lumbar ganglion L2, and ganglion fat were contrasted by high throughput sequencing. The samples that passed QC (Agilent 2100 Bioanalyzer Instrument, RRID:SCR_019389) were processed on Illumina Nextseq 550 (Illumina NextSeq 550 System, RRID:SCR_016381) single-end protocol (75 base pairs).
Completeness: This dataset is a part of a larger study: "Genetically-based neuro-modulation of adipose tissue functions".
Subjects & Samples: Male (n=4) and female (n=4) adult, 5 month old mice (RRID:IMSR_JAX:000664) were used in the study.
Primary vs derivative data: Primary data consists of raw sequencing data in fastq, bz2 files organized in animal ID folders, and tissue sample subfolders.
Important Notes: This dataset is currently undergoing image registration and will be updated once this process is complete.
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