Chemogenetic whole-body and iBAT (interscapular brown adipose tissue) -specific sympathetic stimulation in anesthetized mice

Heike Muenzberg, Ph.D.
,
Clara Huesing, Ph.D.
,
Nathan Lee, B.S.

Functional effects of whole-body and iBAT-specific stimulation while under acute anesthesia

Updated on April 12, 2021 (Version 1)

Corresponding Contributor:

Heike Muenzberg
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Dataset Overview

Study purpose: To examine functional effects of whole-body and iBAT (interscapular brown adipose tissue)-specific stimulation while under acute anesthesia.

Data collection: Blood glucose measurements, rectal temperature, iBAT temperature were collected.

Primary conclusion: iBAT-specific sympathetic activation is permissive, but not sufficient to drive thermogenesis. Sympathetic activation has an inhibitory effect on iBAT temperature that cannot be solely attributed to b3-AR signaling.


Curator's Notes

Experimental Design: Peripheral viral AAV (Adeno-associated virus) injection of 2.5 µL into the right iBAT pad and 2.5 µL injections into the left iBAT (interscapular brown adipose tissue) pad were administered to anesthetized animals. After recovery from the first surgery, the Emitter transponder was implanted to allow for monitoring and recording of physiological data. Blood glucose measurements, rectal temperatures, and iBAT temperatures were collected.

Completeness: This dataset is a part of a larger study: "Genetically-based neuro-modulation of adipose tissue functions".

Subjects & Samples: Male (n=16) and female (n=15) adult, 3-6 months old mice were used in the study.

Primary vs derivative data: Primary data is organized in excel files where physiological recordings are presented in tabular form for all animals used in the study. Subject names are shown internally in each table. There is no derivative data.

Important Notes: This dataset is currently undergoing image registration and will be updated once this process is complete.

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Publishing history

April 12, 2021
Originally Published
April 12, 2021 (Version 1)
Last Updated

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