Chemogenetic iBAT-specific sympathetic stimulation and e-mitter implant
Study purpose: To study genetically-based neuro-modulation of adipose tissue functions.
Data collection: Metabolic data (energy expenditure, respiratory quotient), telemetry data (abdominal (core) temperature, activity), thermal gradient test data, food intake measurement, bodyweight measurement were collected.
Primary conclusion: Our data show the isolated stimulation of iBAT sympathetics results in an acute suppression of body temperature. Even though these data were surprising, they are consistent with our observations to manipulate b3-adrenergic receptors with an antagonist (CL-316,243) or agonist (SR 59230A).
Chemogenetics -/+ b3-adrenergic antagonist: Blockade of b3-adrenergic receptors enhances the suppression of core temperature with chemogenetic activation of iBAT sympathetics, consistent with the idea that isolated activation of iBAT sympathetic (in the absence of iWAT (white adipose tissue) b3-AR activation) has an inhibitory effect on BAT (brown adipose tissue) thermogenesis.
Chemogenetics -/+ b3-adrenergic agonist: Activation of b3-AR receptors enhance the induction of core temperature and weight loss (independent of food intake). Taken together these data suggest that activation of iBAT has a bidirectional function that depends on b3-AR stimulation elsewhere (e.g. stimulation of white adipose tissue depots).
Experimental Design: Peripheral viral AAV (Adeno-associated virus) injection of 2.5 µL into the right iBAT (interscapular brown adipose tissue) pad and 2.5 µL injections into the left iBAT pad were administered to anesthetized animals. After recovery from the first surgery, the Emitter transponder was implanted to allow for monitoring and recording of physiological data.
Completeness: This dataset is a part of a larger study: "Genetically-based neuro-modulation of adipose tissue functions".
Subjects & Samples: Male (n=3) and female (n=3) adult, 5 month old mice were used in the study.
Primary vs derivative data: Primary data is organized in excel files where all metabolic, telemetry, and physiological data is presented in tabular form for all animals used in the study. Subject names are shown internally in each table. There is no derivative data.
Important Notes: This dataset is currently undergoing image registration and will be updated once this process is complete.
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