Optogenetic activation of nitrergic and cholinergic neurons of murine colonic myenteric plexus

Dante Heredia
Thomas Gould

Tension recordings of proximal, middle and distal colon after optogenetic activation of nitrergic and cholinergic myenteric neurons

Updated on April 11, 2021 (Version 1)

Corresponding Contributor:

Thomas Gould
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Dataset Overview

Study purpose: This dataset examines the effect of optogenetic activation of cholinergic and nitrergic myenteric neurons on motility of the colon, measured by simultaneous tension recordings of the proximal (PC), middle (mC), and distal colon (dC).

Data collection: We examined the effect of optically stimulating (stim) cholinergic and nitrergic neurons expressing the light-activated cation channel channelrhodopsin (ChR2) during a period of tonic inhibition, in between spontaneous colonic migrating motor complexes or CMMCs. Tension recordings of proximal, middle, and distal colon after optogenetic activation of nitrergic and cholinergic myenteric neurons were collected.

Primary conclusion: None stated

Curator's Notes

Experimental Design: Optogenetic stimulation of neuronal subtypes in murine colonic myenteric plexus was performed on transgenic mice expressing the optogenetic protein channel rhodopsin-2 in colonic myenteric neurons. The colon was dissected, rod placed within, then three tension transducers were affixed to proximal, middle, distal colon. Tension was initially set at 8 mN, monitored using an MP100 interface, and recorded on a PC running AcqKnowledge Data Acquisition and Analysis Software 3.2.6 (Biopac Systems).

Completeness: This dataset is complete.

Subjects & Samples: Male (n=6) and female (n=6) adult mice (RRID:IMSR_JAX:000664), 8-12 weeks old were used in this study.

Primary vs derivative data: Primary data are organized by subject name and contain files with individual tension recording as raw tracks in AcqKnowledge. The derivative folder contains pdf files for each animal and summary in pptx slides showing tension recording tracks of the 12 animals. Top trace (red) = distal colon (dC), middle (green) = transverse colon (tC), bottom (pink) = proximal colon.

Important Notes: This dataset is currently undergoing image registration and will be updated once this is complete.


This dataset is currently under embargo. Files will be made publicly available on 05/31/2021.

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About this dataset

Publishing history

April 11, 2021
Originally Published
April 11, 2021 (Version 1)
Last Updated

Cite this dataset

This dataset is under embargo and will be made publicly available on 05/31/2021