A detailed topographic map of regional gastric effects on duodenal motility, using duodenal motor responses elicited by gastric stimulation in different regions.
Study Purpose: Recently, gastric electrical stimulation (GES) has been intensively investigated (albeit with little attention to best gastric location) for its therapeutic potential for various GI motility disorders (gastroparesis, eating disorders, etc.). To identify the optimal locations for GES, we generated a detailed topographic map of regional gastric effects on duodenal motility, using duodenal motor responses elicited by gastric stimulation in different regions.
Data Collection: Acute studies were performed with fasted anesthetized rats with patch stimulation electrodes (stimulation parameters: biphasic, I = 0.3mA, pw = 0.2ms, 10 Hz, 20s-on-40s-off, 5 one-minute cycles) sutured onto different stomach sites, with strain gauges attached to the proximal duodenal serosal surface. The strength of duodenal motor activity was quantified as the ratio of various strain gauge signal parameters during/after stimulation vs. before stimulation. Precise locations of stimulation electrode implantation were measured on the removed stomachs at the end of the experiment. We mapped the efficacy of stimulation at randomly assigned electrode placements (n=141) in acute experiments (n=81), and we are using the data to compare to spatial distributions of vagal neurites.
Primary Conclusion: None stated
Curator's Notes
Experimental Design: Measurements of electrical stimulation-induced effects on duodenal motility in young adult Sprague-Dawley rats were taken. Signals recorded from strain gauges attached to the proximal duodenal surface were used to measure the effect of stimulation by patch electrodes implanted at multiple sites across the rat stomach in an acute anesthetized preparation. The effect of stimulation was quantified as the ratio of various motility assessments during and after stimulation vs. before stimulation, and the data was used to create a functional map of duodenal motor response to localized gastric stimulation.
Completeness: this dataset is complete
Subjects & Samples: Male Sprague Dawley rats (n=81) weighting 228‐330 g were used in the study.
Primary vs derivative data: The primary data consists of 141 Excel files (~5MB to ~40MB each) of raw strain gauge data vs. time organized by subject name. All measurements are summarized in the motility data excel file in the derivative folder.
Code availability: In the code folder included is fully commented MATLAB code (main script: MotilityAnalysis_MAIN_forStrainGaugeData_30Oct18.m) to process the raw data to obtain measures of area-under-curve, frequency, amplitude, and an Excel spreadsheet of extracted motility data.
Important notes: This dataset is currently undergoing image registration and will be updated once this process is complete.
0 - 0 of 0 files